siRNA delivery mediated by pH and redox responsive p(DEAEMA-co-HEMA-g-PEGMA) nanogels

Small interfering RNAs (siRNA) have enabled novel, specific, and efficient treatment of diseases, including cancer. To obtain sufficient well-controlled intracellular delivery, a drug delivery system is essential. Herein for siRNA, composed cationic biodegradable nanogels, presented. P(DEAEMA-co-HEMA-g-PEGMA) nanogels with varying ratios 2-diethyl aminoethyl methacrylate (DEAEMA) 2-Hydroxyethyl (HEMA), crosslinked tetraethylene glycol dimethacrylate or disulfide-crosslinker bis(2-methacryloyloxy ethyl), are synthesized. The pH-depended nanogel swelling facilitates siRNA loading endosomal disruption. ensures release by GSH-mediated particle disassociating. within the sub-100nm range in their collapsed state, PDI ≥0.4, ζ-potential ranging from approximately 5-10 swollen state 22–30 V ratio up to 1.6 diameter. Furthermore, show good serum-stability, capable delivering acceptable cytotoxicity levels. induced >90% eGFP-silencing lung cancer cells, gene-silencing target POLR2A led siRNA-induced cell death squamous carcinoma cells. findings suggest that nanogel-system may function as carrier vehicle cytoplasmic siRNA.